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Acid a-Naphthyl Acetate Esterase Stain 100Tests Baso Stains for Medical Diagnosis
Detection Principle
Hydrochloric acid parafuchsin reacts with Sodium Nitrite to form diazonium salt (six-azo-pararosaniline). α-Naphthyl Acetate is decomposed by esterase and produces α-naphthol, which shall combine with diazonium salt to form red brown precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called “Non-specific Esterase Stain.”
Stain Results
Red or red-brown granules found in cytoplasm are positive.
Dot type: It is mainly seen in mature T Lymphocytes. 1~4 brown or red-brown circular, mass, and big spot granules with clear contour in cytoplasm are found in positive reaction.
Diffuse type: Red-brown and dusty granules diffusely spread are found in positive reaction. Granules may appear at a certain part of cell with obscure outline in cytoplasm.
Monocyte type: Evenly stained red-brown granules diffusely spread in cytoplasm are found in positive reaction.
Packaging Specification
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Product Name
| Ref # | Specification |
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Acid a-Naphthyl Acetate Esterase Stain
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BA4112B
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100 Tests/Kit
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